| 초록 |
DNA have attracted attention as the building materials for self-assembled nanostructure. Watson-Cricks base pairing based DNA nano system enables programming of DNA into the various forms of the nanostructure. Over the past decades, DNA have been studied to create from DNA simple structure to DNA supramolecules. Despite these attractions, there is a disadvantage in terms of the high cost for DNA synthesis. Rolling Circle Amplification is a powerful tool that elongate long single-stranded DNA from circular DNA template, containing millions of repeated units. Herein, we report a strategy that produce DNA hydrogel by RCA containing Y and X shaped DNA nanostructures and their separation from the DNA hydrogel with two kinds of restriction enzyme. Because elongated ssDNA contains a restriction site, DNA hydrogel can be digested into Y and X-shaped structures which are rationally designed. Thus, we expect this study offer amplification tool of various complex DNA and RNA nanostructures. |
