| 학회 |
한국공업화학회 |
| 학술대회 |
2021년 봄 (05/12 ~ 05/14, 부산 벡스코(BEXCO)) |
| 권호 |
25권 1호 |
| 발표분야 |
포스터-정밀화학 |
| 제목 |
Gold nanoparticles modified with spore-binding peptides for colorimetric detection of allergenic fungi |
| 초록 |
The rapid and sensitive detection of allergenic fungal spores is of great interest owing to the potentially negative impact of these spores on personal and public health. We present a simple and prompt colorimetric strategy for detecting Aspergillus niger based on interactions between fungal spores and gold nanoparticles (AuNPs) modified with a specific binding peptide. The spore-binding peptide was identified by phage display screening, which exhibited enhanced affinity through multivalent effects. Immobilization of peptide on AuNPs enabled rapid binding to spores, resulting in a visible change in the color intensity of the supernatant after sedimentation of the spores. This simple colorimetric approach displayed a high sensitivity of ~50 spores and a rapid detection time of ＜10 min when used with a smartphone-based image analysis application. |
| 저자 |
이상민, 이정인, 장석천, 정우재
|
| 소속 |
성균관대 |
| 키워드 |
colorimetric sensor; spore-binding peptide; gold nanoparticle; A. niger
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| E-Mail |
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