| 학회 |
한국화학공학회 |
| 학술대회 |
2021년 봄 (04/21 ~ 04/23, 부산 BEXCO) |
| 권호 |
27권 1호, p.491 |
| 발표분야 |
생물화공 |
| 제목 |
Ultrasensitive isothermal amplification method based on nicking and extension chain reaction system for RNA detection |
| 초록 |
We herein developed an ultrasensitive version of nucleic acid sequence-based amplification (NASBA) called Nicking and Extension chain reaction System-Based Amplification (NESBA). The NESBA primer set employed in this technique was designed by incorporating nicking site at the 5’-end of NASBA primer set containing T7 promoter region. The use of NESBA primer set would integrate nicking and extension chain reaction into conventional NASBA system and enable the exponential amplification of T7 promoter-containing double-stranded DNA (T7DNA) through the continuously repeated nicking and extension chain reaction catalyzed by nicking endonuclease (NE) and reverse transcriptase (RT). The amplified T7DNAs are then transcribed to produce a large amount of RNA amplicons which could generate high fluorescence signal through binding to fluorescent molecular beacon probes. With this strategy, we successfully determined the respiratory syncytial virus A (RSV A) genomic RNA (gRNA) with much higher sensitivity than that of conventional NASBA method. |
| 저자 |
문정1, 주용2, 김효용2, 안준기3, 조영란2, 박현규2
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| 소속 |
1한국과학기술원 / 한국생명공학(연), 2한국과학기술원, 3한국과학기술원 / 한국생산기술(연) |
| 키워드 |
생물화학공학 |
| E-Mail |
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| 원문파일 |
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