| 초록 |
Numbers of studies about 3D in vitro cell culture have been introduced due to the enhanced viability and cellular functions. Also, co-culture of different type of cells is demonstrated to be effective for realization of much more in vivo like microenvironments for cells. However, till now, there is a remaining limitation of 3D cell culture that it is impossible to make vascularized tissues, so the in vitro tissues cannot exchange nutrients and metabolites through vessels just as in vivo state. Here, we made co-cultured spheroid of hepatocyte and different types of endothelial cells in 10:1 ratio and re-suspended the spheroids in collagen gel for making sprouting vessels in micro-scale tissues. We compared hepatic function of each group in vitro, and implanted the micro tissues in subcutaneous area of nude mice after 50% hepatectomy to figure out the rapid engraftment of implanted tissue and the possibility to be used as implantable liver tissue during liver regeneration. |
