학회 | 한국공업화학회 |
학술대회 | 2019년 가을 (10/30 ~ 11/01, 제주국제컨벤션센터(ICC JEJU)) |
권호 | 23권 2호 |
발표분야 | 포스터_생물공학 |
제목 | E. coli co-culture and resveratrol production with 13c-MFA |
초록 | 13C-MFA (Metabolic Flux Analysis) is a technique for decoding fluxes of central carbon metabolism using a C source marked 13C. Provide a C source marked 13C in strain and measure the label information of the metabolite. It is then possible to track label information based on a model that decodes flux using specific enzymes in the strain. Resveratrol is a beneficial compound found in plants. It has the effects of cancer, antioxidants and life extension. Resveratrol can be created using Acyl-CoA in the EMP pathway and Tyrosine in the PP pathway. However, the road has a different temper and is difficult to turn into a single strain. Thus, an attempt was made to produce resveratrol using a co-culture. In this study, two E. coli were designed to supply more resveratrol precursor based on previous studies. Then we will produce resveratrol with a co-culture. Finally, using 13C-MFA to analyze the metabolic rate of co-culture, and ways to increase resveratrol will be discussed. |
저자 | 이준민, 홍재승, 오민규 |
소속 | 고려대 |
키워드 | 13C; resveratrol |