화학공학소재연구정보센터
학회 한국공업화학회
학술대회 2019년 가을 (10/30 ~ 11/01, 제주국제컨벤션센터(ICC JEJU))
권호 23권 2호
발표분야 포스터_생물공학
제목 E. coli co-culture and resveratrol production with 13c-MFA
초록 13C-MFA (Metabolic Flux Analysis) is a technique for decoding fluxes of central carbon metabolism using a C source marked 13C.
Provide a C source marked 13C in strain and measure the label information of the metabolite. It is then possible to track label information based on a model that decodes flux using specific enzymes in the strain.
Resveratrol is a beneficial compound found in plants. It has the effects of cancer, antioxidants and life extension.
Resveratrol can be created using Acyl-CoA in the EMP pathway and Tyrosine in the PP pathway. However, the road has a different temper and is difficult to turn into a single strain.
Thus, an attempt was made to produce resveratrol using a co-culture.
In this study, two E. coli were designed to supply more resveratrol precursor based on previous studies. Then we will produce resveratrol with a co-culture. Finally, using 13C-MFA to analyze the metabolic rate of co-culture, and ways to increase resveratrol will be discussed.
저자 이준민, 홍재승, 오민규
소속 고려대
키워드 13C; resveratrol
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