| 초록 |
Colon cancer is the third common cancer in the United States due to metastasis. In this work, we aimed to develop a targeted probes for detecting colon cancer by using peanut lectins (PNA) conjugated to hyaluronate (HA) with preferential binding to tumor tissues. The HA-PNA conjugate was successfully synthesized by the coupling reaction between aldehyde modified HA and the N-terminal amine group of PNA. For the diagnostic application, rhodamine B (Rho B) was conjugated onto the PNA of HA-PNA conjugates. The intracellular delivery of HA-PNA conjugates to colon cancer cells was confirmed by confocal microscopy. After injection of HA-PNA-Rho B conjugates into the tumor-bearing mice, colon tumor was visualized by optical imaging. Ex vivo two-photon microscopy visualized more efficient delivery of HA-PNA-Rho B conjugates to dysplastic lesions than normal cells. Taken together, we could confirm the feasibility of HA-PNA conjugate as targeted probes for the detection of colon cancer. |
