| 학회 |
한국공업화학회 |
| 학술대회 |
2017년 봄 (05/10 ~ 05/12, 광주 김대중컨벤센센터(Kimdaejung Convention Center)) |
| 권호 |
21권 1호 |
| 발표분야 |
생체재료 |
| 제목 |
Preparation and Evaluation of Ternary complexes with pDNA, Chitosan-g-PEI and Hyaluronic acid for Targeted Colon Cancer |
| 초록 |
Branched polyethyleneimine (PEI) was well known as high gene transfection agent, However, utilization of PEI as gene carrier was limited because of high cytotoxicity. To solve this problem, PEI was introduced to chitosan (CS) back bone by coupling agent. Also, hyaluronic acid(HA) was introduced for targeted CD44-receptor in colon cancer cell. Structure of PEI-grafted chitosan (CSPEI) was analyzed by 1H-NMR. In addition, ternary complex of pDNA/CSPEI/HA was prepared by electrostatic charge interaction and gel retardation assay showed that pDNA was completely condensed by CSPEI and HA. Moreover, pDNA was protected from DNase. Also, transfection assay and cellular uptake showed that ternary complexes were enhanced more than binary complexes by targeting effect of HA. These results suggest that it can be enhance to efficacy of gene delivery in colon cancer cell. (No. NRF-2014R1A2A1A10053027). |
| 저자 |
홍웅길1, 최창용2, 정경원1, 안준혁1, 나재운1
|
| 소속 |
1순천대, 2한영대 |
| 키워드 |
Ternary complexes; Targeted CD44-receptor; Transfection; Colon cancer
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| E-Mail |
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