| 초록 |
Research on fuel production through biological conversion of C1 gas is drawing attention. Since this technology uses greenhouse gases such as CO and CO2, it has both environmental and economic benefits. In this study, clostridium autoethanogenum was used to convert CO into ethanol and acetate in the continuous stirred-tank reactor using two-stage process. In stage I, the reactor for high concentration cultivation, the culture broth was diluted at for not to accumulate products which are toxic to the cell. In stage II, the reactor for high concentration production, continuous cultivation had allowed the product to be diluted steadily, and fresh cells were steadily supplied from the stage I, which allowed for sustainable long-term production of bio-ethanol. As a result, the two-stage process was successfully operated for 58 day. Maximum optical density was 49.48 of OD600 in 58 day. Maximum ethanol and acetate concentration reached 41.18 g/L and 27.49 g/L respectively. |
