| 학회 |
한국화학공학회 |
| 학술대회 |
2011년 가을 (10/26 ~ 10/28, 송도컨벤시아) |
| 권호 |
17권 2호, p.2117 |
| 발표분야 |
환경 |
| 제목 |
Isolation of xylanolytic bacteria from agricultural resources and cloning of xylanase gene |
| 초록 |
Eleven microorganisms, expected to be xylanolytic microorganisms were isolated fromagricultural resources, such as soil of sweet sorghum field, compost, decayed sweet sorghum and decayed wood. These strains were screened at 35℃ by using plate count agar containing 1% (w/v) xylan (from the beech wood) instead of glucose. Among the strains, SS8 and SS10 microbial strains, which have high xylanase activities, were characterized and identified as Staphylococcus sp. by analysis of 16S rDNA sequence and biochemical studies, and named as Staphylococcus sp. SS8 and SS10, respectively. The xylanase gene was cloned from SS8 and SS10 genomic DNA by polymerase chain reaction (PCR). The amplified PCR products were ligated with the T&A cloning vector system and the constructed plasmids were transformed into E. coli DH5α. The sequence analysis of the insert DNAs revealed the identification of a 640-bp region containing xylanase open reading frame. According to xylanase gene sequence analysis, both strains had gene sequence similarity of 99% with Bacillus subtilis Xyl gene for xylanase (AB457186.1). |
| 저자 |
김중곤, 안승현, 박유리, 차영록, 안기홍, 박선태, 문윤호, 최용환, 구본철, 박광근
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| 소속 |
농촌진흥청 국립식량과학원 바이오에너지 작물센터 |
| 키워드 |
xylanolytic bacteria; xylanase; Staphylococcus sp.
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| E-Mail |
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| 원문파일 |
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