| 초록 |
Synergistic combination of gene targeting and chemotherapy by co-delivering siRNA and anticancer drugs has widely been investigated to develop siRNA-based therapeutics for cancer treatment. . Here we present a simple and biocompatible co-delivering formulation based on a unique complexation method, i.e., multiple monocomplexation iduced hydrophobic association between siRNA and a monocationic anticancer agent . A colloidal formulation of the "HMplex" composed of siRNA, and Pluronic was spontaneously constructed by physical mixing of the ternary constituents. In vitro and in vivo studies revealed that the HMplex with a lower charge ratio possesses a tightly complexed stable nanostructure with Pluronic surface and small colloidal size around 10 nm, which allowed for 1) suitable protection of siRNA in serum-rich physiological environment, 2) efficient intracel-lular transfection into the cytoplasm, and 3) successful co-delivery into the tumor tissue with dense interstitial matrix. |
