| 학회 |
한국화학공학회 |
| 학술대회 |
2008년 가을 (10/23 ~ 10/24, 부산 BEXCO) |
| 권호 |
14권 2호, p.2729 |
| 발표분야 |
생물화공 |
| 제목 |
Fabrication of high density and activity protein array using micropatterned dendrimer |
| 초록 |
In this study, we prepared a high-density microarray of porphyrin dendrimer terminated with anions (COONa-) which can provide multiple functional sites for proteins to attach, and thus increase the sensitivity for detection. Substrates were modified with silane group (3-aminopropyltriethoxysilane, APTES) followed by one was modified with aldehyde group and the other was modified with dendrimer. Compared with aldehyde group, protein loading and activity in modifying dendrimer was about 2 times greater. Micropatterns of dendrimer were prepared on silicon substrates modified with APTES by contacting polydimethylsiloxane (PDMS) stamp with 200 μm or 300 μm pillar diameter. After removing PDMS stamp from the substrates, well-defined dendrimer microspatterns were obtained. Proteins were immobilized on the individual patterns modified with N-hydroxysuccinimide (NHS) and 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) and florescent microscopy proved that proteins were successfully attached within a micropattern. Based on these results, the biotinstreptavidin system was investigated and the molecular recognition mediated, specific binding between biotin and streptavidin was successfully assayed, demonstrating the possibility of micropatterned dendrimers for various biosensor. |
| 저자 |
이 열, 김대년, 박상필, 장은지, 박새미, 김종수, 고원건
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| 소속 |
연세대 |
| 키워드 |
Dendrimer; Micropatterning; PDMS Stamp; Protein
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| E-Mail |
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| 원문파일 |
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