| 학회 | 한국화학공학회 |
| 학술대회 | 2009년 봄 (04/23 ~ 04/24, 광주 김대중컨벤션센터) |
| 권호 | 15권 1호, p.39 |
| 발표분야 | 고분자 |
| 제목 | Development of phenol detecting biosensor utilizing quantum dots and poly(ethylene glycol) hydrogel |
| 초록 | Phenol is an important but toxic starting material in a broad range of chemical manufacturing processes. Soil and surface water of areas around formal production and processing plants are often contaminated by phenol with risk to ground water resources. In this study, a simple and sensitive detection method for phenols were investigated using poly(ethylene glycol) (PEG) hydrogel microparticles entrapping quantum dot (QD) and tyrosinase enzyme.Hydrogel microparticles were fabricated by using dispersion polymerization or photopatterning methods, and size of resultant particles was ranged from 10 to 300 μm.The enzymatic reaction used for the detection of phenol is the oxidation of phenolic compounds via tyrosinase. In such reaction, phenol is oxidized to o-quinone which is a classic electron accepting chemical that can quench the fluorescence of QDs.Accuracy of the system was verified by measurement of photoluminescence (PL) quenching in free solution state with different concentrations of phenol. The PL quenching of QDs were determined by the molecular weight of PEG, and concentration of QDs and/or enzymes. |
| 저자 | 장은지, 이 열, 김대년, 박상필, 박새미, 남승희, 이현종, 고원건 |
| 소속 | 연세대 |
| 키워드 | Hydrogel; Quantum Dot; Phenol; Tyrosinase |
| 원문파일 | 초록 보기 |
