| 초록 |
L-tyrosine is a commercially important compound in the food, pharmaceutical, and cosmetic industries. Microbial production of L-tyrosine is attractive, but it still remain to solve the problems for improving L-tyrosine production such as metabolic flux amplification and carbon flux rebalancing around phosphoenolpyruvate (PEP) node. Here, we constructed L-tyrosine high producing strain based on synthetic biology approach. To amplify the metabolic flux of L-tyrosine biosynthetic pathway, a synthetic constitutive promoter and a synthetic 5′-untranslated region were introduced for each gene to allow for maximizing the expression level. Carbon flux rebalancing was achieved by controlling the expression level of PEP synthetase using UTR engineering based on UTR Designer. The L-tyrosine productivity of the engineered strain was improved resulting in 3.0 g/L of L-tyrosine titer, 0.0354 g L-tyrosine/h/g DCW of productivity, and 0.102 g L-tyrosine/g glucose yield. |
