| 학회 |
한국공업화학회 |
| 학술대회 |
2015년 봄 (04/29 ~ 05/01, BEXCO (부산)) |
| 권호 |
19권 1호 |
| 발표분야 |
생체재료_포스터 |
| 제목 |
Polydiacetylene immobilized surface-enhanced Raman scattering-encoded beads for label-free detection |
| 초록 |
An understanding of protein–protein interactions can be obtained from multiplex protein detection methods, and spectrally coded beads can provide efficient means for this type of detection. In this study, we report that polydiacetylene- coated surface-enhanced Raman scattering-encoded beads (PDA–SERS beads) which can provide an encoding capacity owing to their SERS properties as well as for label-free detection from the PDA layer. We also introduced ligands to the PDA–SERS beads which enabled the detection of target proteins by PDA fluorescence. The fluorescence intensities of biotin-conjugated PDA–SERS beads were increased with an increase in the concentration of streptavidin by using PDA–SERS beads immobilized with biotin. We could detect 2 x 10-8 M of streptavidin by measuring the fluorescence intensity without an additional labeling step. |
| 저자 |
전봉현 |
| 소속 |
건국대 |
| 키워드 |
PDA (polydiacetylene); surface-enhanced Raman scattering (SERS); label-free detection; colorimetric detection
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| E-Mail |
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