| 학회 | 한국고분자학회 |
| 학술대회 | 2003년 가을 (10/10 ~ 10/11, 부경대학교) |
| 권호 | 28권 2호, p.169 |
| 발표분야 | 의료용 고분자 부문위원회 |
| 제목 | Preparation and biological effect of gelatin-κ-carrageenan scaffolds for cultured artificial dermal substitute |
| 초록 | Skin substitutes with living cells have been promptly produced by in vitro three-dimensional culture techniques. These techniques promise a new approach to the repair and reconstruction of tissue damaged by burn injury, mechanical injury and pressure sores. Natural polymers have low mechanical strength compared with synthetic polymers. For this reason, κ-carrageenan was induced to make the scaffold strong. The objective of the present study was to prepare the porous gelatin-κ-carrageenan scaffolds and to investigate their cell attachment, morphological characteristics and wound healing effects. In experiments, scaffolds were manufactured by freeze-drying and crosslinked by 1-ethyl-(3-3-dimethylaminopropyl) carbodiimide hydrochloride (EDC). The scaffolds showed the inter-connected pore structure. In biodegradation in vitro, the degradation rate increased with porosity of scaffold. Also, it was found that gelatin- κ-carrageenan sponge has enough mechanical properties for cell culture and transplantation for artificial skin. To prepare the cellular dermal substitutes, the fibroblasts isolated from child foreskin were cultured in the gelatin- κ-carrageenan scaffolds. Results on cell attachment revealed that the high gelatin content was suitable for cellular attachment and distribution in three-dimensional fibroblast culture. |
| 저자 | 임윤묵1, 김용한1, 정무상1, 이상봉1, 이영무1, 노영창2 |
| 소속 | 1한양대, 2한국원자력(연) |
| 키워드 | gelatin; carrageenan; artificial skin; scaffold |
