| 초록 |
For the regeneration of injured skeletal muscle tissue, stem cells have been studied as a source of replacing damaged tissue. In recent years, it has been demonstrated that adipose-derived stem cells (ASCs) can be differentiated into myogenic lineage, then fused into myotubes when they are co-cultured with myoblasts. In addition, topographically aligned scaffold is also important to mimic the native structure of skeletal muscle tissue. In this study, we co-cultured ASCs and C2C12 myoblasts on aligned nanofiber scaffold. This scaffold is fabricated by electrospinning poly(caprolactone) (PCL), which is a biocompatible material. Then, it is patterned with poly(ethyleneglycol) (PEG) hydrogel for easy handling and distinguishing cell seeding areas. ASCs and C2C12 myoblasts are blended and seeded onto the scaffold, and then treated in differentiation medium. This co-culture system integrated with topographical cue is expected to be potentially applied to skeletal muscle tissue engineering. |
