| 초록 |
Nucleic acid nanotechnology has drawn tremendous attention, since it can provide a precise tool for building multi-dimensional structures with a defined size, shape, and surface property. Here, we show that self-assembled functional nucleic nanostructures with a well-defined size can serve as a multi-functional platform to induce efficient RNA interference, mRNA expression, and CRISPR/Cas9 mediated gene editing. For the synthesis of functional RNA structures, we have utilized the rolling circle transcription of pre-designed DNA template to produce a large quantity of functional RNAs. For programmable and simultaneous gene silencing, we have design the dicer substrate RNA nanostructures to regulate three different fluorescent proteins (GFP, RFP, and BFP) in the target cells. Using three arm junction RNA nanostructures, we were able to show programmable regulation of fluorescent protein expression to generate 8 different fluorescent colored cells. |
