| 학회 |
한국고분자학회 |
| 학술대회 |
2021년 가을 (10/20 ~ 10/22, 경주컨벤션센터) |
| 권호 |
46권 2호 |
| 발표분야 |
대학원생 구두발표(영어발표, 발표15분) |
| 제목 |
Clustered CTLA-4 siRNA and CpG nanoplexes for cancer immunotherapy |
| 초록 |
Cancer immunotherapy taking the advantages of adaptive immunity have gained increasing interests because it not only suppresses tumor development but induce generation of immunological memory cells and prevent tumor regeneration. Here, we fabricated nanoplexes (NP) composed of aizdo-PEG grafted branched polyethylenimine and CTLA-4 siRNA (siRNA NP) or CpG1826 (CpG NP) by electrostatic interaction. The two NPs were associated into nanoplex clusters (siRNA/CpG NCs) via click chemistry using an alkyne terminated MMP-2-cleavable peptide as a linker. The CpG NPs was demonstrated considerable BMDC activation with increased CD80 and CD86 surface expression. Likewise, splenocytes treated with siRNA NPs showed significant down-regulation of CTLA-4. When administered in vivo, the siRNA/CpG NCs was able to accumulated in tumor and be cleaved by MMP-2 overexpressed in tumor site. The dissociated NPs were released CpG and siRNA when uptaken by cells and activate immune system to combat tumors. |
| 저자 |
모미, 유혁상, 박재근, Oanh-Vu Pham-Nguyen, 이미소, 조완호
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| 소속 |
강원대 |
| 키워드 |
Immunotherapy; CpG ODN; CTLA-4 siRNA
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| E-Mail |
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