| 초록 |
Bioethanol is the most common renewable fuel and a up-coming chemical feedstock. As a source for bioethanol production, a promising non-food biomass, cellulosic biomass is composed of cellulose, hemicellulose and lignin. By its decomposition, various mono-sugars are released. Among them, most abundant sugars of glucose and xylose should be fermented to bioethanol for gainingits price competitiveness against petroleum-oriented fuels and chemicals. In this study, a traditional alcohol fermenting yeast, Saccharomyces cerevisiae, was engineered to produce bioethanol from glucose and xylose efficiently. Because S. cerevisiae is unable to use xylose as a sole carbon source and highly sensitive to toxic compounds in cellulosic biomass hydrolyzates, microbial factory technology armed with omics technology, metabolic engineering, cofactor engineering and process optimization was chosen for overcoming such technical hurdles. |
