| 학회 |
한국공업화학회 |
| 학술대회 |
2017년 봄 (05/10 ~ 05/12, 광주 김대중컨벤센센터(Kimdaejung Convention Center)) |
| 권호 |
21권 1호 |
| 발표분야 |
생물공학 |
| 제목 |
High-throughput N-glycan analysis using a 96-well plate platform by MALDI-TOF-MS |
| 초록 |
In human glycoproteins, N-glycans are the major constituents of the outer cell-membranes and have a crucial role in biological and immunological systems. This work has focused on a rapid high-throughput analysis of N-glycans using a 96-well plate platform based on MALDI-MS. Although the chemical derivatization processes were performed in a 96-well with built-in 10 kDa MWCO membrane filters, the quantitative linearity was still quite good between varying amounts of the target glycoprotein and the MALDI peak intensities. In addition, we validated the relative quantitative reproducibilities in different well positions in a 96-well plate. As a proof-of-concept, the proposed HT MALDI-QTaG method was successfully used to analyze bovine fetuin, human serum and Enbrel. |
| 저자 |
황철환1, 박한규1, 송원석2, 안다희1, 정윤이1, 김성민1, 김윤곤1
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| 소속 |
1숭실대, 2서울대 |
| 키워드 |
N-glycan; MALDI-MS; UPLC-FLR; High-throughput analysis
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| E-Mail |
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