| 학회 |
한국고분자학회 |
| 학술대회 |
2012년 가을 (10/11 ~ 10/12, 창원컨벤션센터) |
| 권호 |
37권 2호 |
| 발표분야 |
Smart Carriers for Bioactive Molecules and Cells(의료용 고분자 부문위원회) |
| 제목 |
Bio-reducible nanocarrier for therapeutic microRNA delivery to the treatment of breast cancer |
| 초록 |
Polysorbitol-based osmotically active transporter (PSOAT) was shown to possess dramatically enhanced transfection activity with reduced cytotoxicity. miR-145 expression levels are usually down-regulated in breast cancer tissues than in their normal counterparts. PSOAT was utilized to deliver microRNA 145 for the treatment of breast cancer. The miR-145 and GFP dual expression reporter plasmid was delivered with bio-reducible PSOAT gene carrier to study the transfection efficiency. The effects of the expressed miR-145 on proliferation, migration and invasion in vitro were analyzed. The repression of the targets of the miR-145 and the downstream signaling pathway was studied with PCR and western blot analysis. The tumor xenograft model was established, and the PSOAT complexed miR-145 was intra-tumorally injected. The tumor suppression after the delivery of particulate microRNA-145 was studied and confirmed with immunohistochemical analysis. |
| 저자 |
MUTHUNARAYANAN MUTHIAH, 차혜련, 박인규
|
| 소속 |
전남대 |
| 키워드 |
Bio-reducible; miRNA; Breast cancer
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| E-Mail |
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