| 학회 |
한국화학공학회 |
| 학술대회 |
2001년 가을 (10/19 ~ 10/20, 한밭대학교) |
| 권호 |
7권 2호, p.3967 |
| 발표분야 |
생물화공 |
| 제목 |
배양 pH가 한세눌라 폴리모르파의 균체성장과 재조합 인체 표피 성장인자 고유 생산에 미치는 영향 |
| 초록 |
The pH dependency of cell growth and human epidermal growth factor (hEGF) production was studied with a recombinant Hansenula polymorpha strain. Maximum specific growth rate and apparent specific production rate of hEGF was highly dependant on culture pH over the range of 4.0-7.0. The optimal pH for the fastest growth was 5.0 and for the apparent production of hEGF 6.0. A simple kinetic model considering the degradation of hEGF during culture was established and then used to estimate the intrinsic specific production rate of hEGF according to culture pH. The intrinsic specific production rate of hEGF was relatively unaffected by culture pH over the range of 5.0-7.0. An optimum hEGF production strategy of pH shift was suggested for the two-phase culture of methylotrophic yeast. |
| 저자 |
문현수, 이상기, 최의성, 김승욱, 김익환, 홍석인
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| 소속 |
고려대 |
| 키워드 |
hEGF; pH; degradation; intrinsic production
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| E-Mail |
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| 원문파일 |
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