| 초록 |
Delivery of small interference RNA (siRNA) can achieve the inhibition of target gene expression in cancer cells. Different from chemical anti-cancer drugs, it has low cytotoxicity when delivered to human body and effectively suppress the gene expression of cancer cells. On the other hand, the delivery of siRNA needs to overcome several limitations related with the nature of RNA. The degradation of RNA by ribonuclease in human body is the one of critical difficulty for the delivery of siRNA. Here, to address these issues, we describe a novel enzymatically synthetic method to generate self-assembled RNA nanoparticles with DNA aptamer for carrier-free siRNA delivery. By novel rolling circle replication method, we can successfully produce the nucleic acid-based structures composed of DNA-RNA hybrids. Furthermore, RNA and DNA strands are rationally designed to contain siRNA precursors and aptamers that bind specifically to the target cell-surface receptor, respectively. |
