| 초록 |
The RNA-guided nuclease CRISPR (clustered regularly interspaced short palindromic repeat)/CRISPR-associated (Cas) protein 9 system is a new emerging genetic material in the field of gene therapy. However, efficiency of genetic edition of target gene using Cas9 protein and gRNA remains to be fully realized. In this study, we prepared the Cas9/gRNA cluster loaded to Y-RNA structure to incorporate multiple sgRNAs for multiplex editing. By loading siRNA of eGFP, tagBFP, and dsRed on each arms of Y-RNA structure, we can achieve delivery of siRNA of eGFP, tagBFP, and dsRed simultaneously to MEF cells, which co-expresses all these three proteins. The resultant cells repress the expression of eGFP, tagBFP, and dsRED. |
