| 초록 |
The present work investigates the properties of terpolymeric hydrogel of hyaluronate (HA) as a cyto-compatible biomaterial with high mechanical properties via conventional free radical polymerization technique. To improve the mechanical properties, 2-hydroxyethyl acrylate (HEA) was attached in HA, while to enhance the cell attachment and proliferation, gelatin methacryloyl (GM) was used as a cross-linker. The chemical modifications were characterized using FTIR and 1H-NMR, whereas the thermal and mechanical properties were investigated using TGA and texture analysis, respectively. The surface morphology of the dried hydrogels was observed under SEM which showed highly interconnected porous structures suitable for cell attachment and proliferation. The cytotoxicity effects of the produced hydrogels were tested in vitro using chondrocytes cells through common cytotoxicity assays. Further in vivo immunogenic response of the hydrogels was studied in a mouse model by implanting subcutaneously. Also, the ability of the hydrogels to load and release different proteins and drugs in a sustained way was demonstrated using a model protein and drug, bovine serum albumin and corticosteroid drug-dexamethasone, respectively at normal physiological conditions in vitro. On whole, the exceptional mechanical properties, interconnected porous structure, stability, biocompatibility, sustained release properties of the HA-g-pHEA-x-GM hydrogel shows its high applicability in cartilage tissue engineering and drug delivery. (1) Das et al., Carbohydr. Polym., 207, 628 (2019). |
