| 학회 |
한국고분자학회 |
| 학술대회 |
2021년 가을 (10/20 ~ 10/22, 경주컨벤션센터) |
| 권호 |
46권 2호 |
| 발표분야 |
기능성 고분자 |
| 제목 |
Integration of a Fiber-based Cell Culture and Biosensing Platform for in situ Monitoring of Protein Markers Secreted from Stem Cells |
| 초록 |
We propose a new platform that can integrate three-dimensional cell culture scaffold and a surface-enhanced Raman spectroscopy (SERS)-based biosensor by stacking them to form a multilayer system, which would allow in situ monitoring of the protein markers secreted from cultured stem cells without periodic cell and/or media collection. The cell culture scaffold supported the proliferation and osteogenic differentiation of adipose-derived mesenchymal stem cells. The SERS capture substrate detected protein markers in combination with SERS tag made with Au-Ag alloy nanoboxes. Incorporating the different Raman reporters into the SERS tag allowed easy identification of target proteins for multiplex assays. When one ADSC culture scaffold and multiple SERS capture substrates were integrated in differentiation culture media, our system was sensitive to monitor time-dependent secretion of three different osteogenic protein markers from ADSCs during their osteogenic differentiation. |
| 저자 |
고재한1, 함정우2, 이화정2, 김태현2, 이강원1, 고원건2
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| 소속 |
1서울대, 2연세대 |
| 키워드 |
Integrated cell culture-biosensor platform; SERS; Electrospun fibers; Cell-secreted biomarker; Stem cell differentiation
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| E-Mail |
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