| 초록 |
AuNPs were Synthesized by using di- or tri-carboxylate-PEG copolymers, including citrate-PEG(CPEG), malate-PEG (MAP), and tartrate-PEG(TAP), as a reducing and stabilizing agent. The freely available Hydroxyl or carboxylate groups of CPEG, MAP, and TAP were used to attach folate (FA), via an EDC/NHS coupling reaction to obtain FA-CPEG-AuNP, FA-MAP-AuNP, and FA-TAP-AuNP nanoconjugates, respectively. The cancer drug 5-fluorouracil (5FU) attached to the π back-boned carbonyl oxygens of the nanoconjugates, and the in vitro drug release profile was studied by high pressure liquid chromatography. The polymer- gold nanoconjugates exhibited sustained release of 5FU up to 27days. FA-MAP-AuNPs exhibited an IC50 value of 5μg/ml, while FA-CPEG-AuNPs and FA-TAP-AuNPs showed IC50 values of 100 μg/ml towards MCF-7 cells. |
