| 초록 |
Many researchers have studied the employment of electrospun fibers to encapsulate and immobilize large amounts of drug on expansive surface areas. In this study, we prepared a DNA-loaded nanofibrous matrix for epidermal gene delivery. For controlled release of DNA loaded in the matrix, a matrix metalloproteinases (MMPs)-cleavable linker was introduced between cationic polymer, linear poly (ethyleneimine) [LPEI] and the matrix so that external MMPs can cleave the linker to release LPEI and DNA. Many clinical investigations have shown that diabetic patients express higher concentrations of MMPs, including MMP-2 and MMP-9, in foot ulcers, thus leading to an attenuated wound recovery process. Thus, exposure to higher levels of MMPs during the diabetic wound healing process was expected to accelerate release of the LPEI/DNA complex from the nanofibrous matrix. |
