| 초록 |
In this study, we reports a Clostridium strain revealing a distinct reduction peak in cyclic voltammogram when three electrodes with grassy carbon electrode (working electrode), platinum electrode (counter electrode), and Ag/AgCl reference electrode were immersed into the culture of the Clostridium strain. This electroactive behavior of the strain supports the direct electron transfer from a cathode to the strain. From further analysis, we found increasing NADH/NAD+ ratio, an intracellular factor representing a reducing power for the fermentation. CO2 reduction was tested using the electroative strain poised at -160 mV vs. Ag/AgCl under CO2:H2 (2:8) gas. Interestingly, an area of unknown peak increased over time on a high performance liquid chromatography, whereas there was no peak without an electrical power, implying the biotransformation of CO2 by the strain using electrons from the cathode. Further study is ongoing to identify an unknown product using gas chromatography/mass spectrometry (GC/MS) and searching candidates using the GC/MS library. |
