| 초록 |
Vectors are the most important component for gene therapy. The viral vectors are widely used because of their higher transfection efficiency. However, viral vectors have limitation of genetic material size, oncogenic potential and immunogenicity. Thus, the non-viral vectors have been developing to substitute viral vectors. In this study we utilized carbon dots (c-dots) as a non-viral vector and c-dots have unique properties such as great water solubility and low toxicity. The c-dots were generated using cationic polymer for gene delivery. The therapeutic gene complexed with c-dots (gene/c-dots) were transfected into human mesenchymal stem cells (hMSCs). C-dots have been found to be safer because c-dots are less toxic than cationic polymer. In addition, c-dots enable bioimaging after transfection of gene/c-dots into hMSCs (gene/c-dots@hMSCs). Even, the amount of secreted therapeutic protein from gene/c-dots@hMSCs was 25-fold higher than that from gene/cationic polymer@hMSCs. |
