| 초록 |
For the regeneration of injured skeletal muscle tissue, adipose-derived stem cells (ADSCs) have been studied because they have potential to be differentiated into myogenic lineage. Topographically aligned scaffold is also an important cue to induce myogenesis of ADSCs because it mimics the native structure of skeletal muscle tissue which is highly aligned. In this study, we cocultured ADSCs and C2C12 myoblasts on aligned nanofiber scaffold. This scaffold is fabricated by electrospinning poly(caprolactone) (PCL), which is biocompatible material. Then, it is patterned with poly(ethylene glycol) (PEG) hydrogel for easy handling and distinguishing cell seeding area. ASCs and C2C12s are blended and seeded onto the scaffold, and then treated in differentiation media. This coculture system integrated with topographical cues is expected to be potentially applied for skeletal muscle tissue engineering. |
