| 학회 |
한국고분자학회 |
| 학술대회 |
2018년 가을 (10/10 ~ 10/12, 경주컨벤션센터) |
| 권호 |
43권 2호 |
| 발표분야 |
고분자가공/복합재료 |
| 제목 |
Sensitive multiplexed Immunoassay using Silica coated gold nanoparticle based on MEF effect in shape-coded hydrogel |
| 초록 |
MEF has been widely studied to improve the sensitivity of protein based bioassays. MEF is a well-known technology, wherein at the certain distance between metallic nanoparticle and fluorophores result in fluorescence enhancement. Silica coated goldnano particle is a promising candidate for fluorescence biosensing owing to their high enhanced-fluorescence intensity and low toxicity. In addition, hydrogel is the three dimentional structured soft and elastic material that absorbs water providing biological enviroment. In our study, AuNps were coated with different thickness of silica to optimize the MEF effect. We also prepared different shapes of PEG hydrogel microparticles for multiplexed suspension immunoassaays. After confirming immobilization of IgG on the surface of maximized MEF effect SiO2@AuNPs, entrapped in the different shape of hydrogel microparticles. It is expected to utilize for high sensitive multiplexed immunoassay detecting various analytes. |
| 저자 |
이재상, 김경주, 노소영, 공혜연, 이경민, 고원건
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| 소속 |
연세대 |
| 키워드 |
metal-enhanced fluorescence; silica coated gold nanoparticle; hydrogel; suspension immunoassay
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| E-Mail |
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