| 초록 |
GHK, a naturally occurring copper (II)-chelating tripeptide is known to have activities in wound healing, anti-inflammation, and collagen stimulation. Various reports demonstrate the recovering effects of GHK (with or without copper) in skin stem cells, and skin fibroblasts, which makes this unique peptide a reparative ingredient in cosmetics. In this research, HA (hyaluronic acid) which is part of the ECM (extracellular matrix), was used as a GHK storing depot. The tripeptide was merged into an amphiphilic 8-mer peptide GHKYYXYY (X=A, C), where the tyrosine could be cross-linked to HA-Ty (tyramine-conjugated HA) to form a hydrogel. Horseradish peroxidase (HRP) was used as a cross-linking agent of HA and peptides. When applied to skin wounds, the hydrogel is expected to adhere on the injured tissue and GHK could exhibit wound healing effects without washing out. |
