| 초록 |
Immune checkpoints play an important role in maintaining the balance between activation and inhibition status of human immune functions. In particular, PD-1 and its ligand, PD-L1, are well-known receptors to control the T cell activity, and it has been well documented that their abnormal regulation is highly connected to progression of multiple diseases such as cancer, infection, and autoimmune diseases. Recently, the soluble form of PD-L1 (sPD-L1) has been detected in the serum of patients. However, its detailed biological functions and potential applications for therapeutic or diagnostic purposes remain elusive. In this study, we engineered the ectodomain of human PD-L1 for improved PD-1 binding, and characterization of the isolated human PD-L1 variants are in progress. Biochemical analyses were conducted, and we will discuss the results and their potential applications. |
