| 학회 |
한국고분자학회 |
| 학술대회 |
2018년 봄 (04/04 ~ 04/06, 대전컨벤션센터) |
| 권호 |
43권 1호 |
| 발표분야 |
의료용 고분자 부문위원회 I |
| 제목 |
Promotion of Vascular Formation of Endothelial Cells Co-cultured with Human Adipose-derived Mesenchymal Stem Cells using Polycaprolactone/Gelatin Nanofibrous Scaffolds |
| 초록 |
Blood vessel is essential process for tissue regeneration to deliver oxygen and nutrients and maintain tissue metabolism. Here we developed a technique to reconstruct microvascular system using polycaprolactone (PCL) and gelatin based nanofibrous structure and co-culture system. Using simple electrospinning process, we fabricated 3D mesh scaffold to support endothelial cells (ECs) sprouting along the electrospun nanofiber, and co-culture with adipose-derived mesenchymal stem cells (ADSCs) supporting greater sprouting of ECs. The vascular formation in our system showed more effective paracrine signal from ADSCs to assist vascular formation of ECs than influence of growth factor. Co-cultured ECs expressed higher level of vascular markers (PECAM-1, podocalyxin). We established a bioengineered 3D culture system to promote fast and functional vascular. Co-culture system in nanofibrous scaffold can be applied in the future for restoration of blood vessel. |
| 저자 |
국윤민1, 김혜림2, 김수진2, 허찬영3, 박민희2, 이강원2, 고원건1
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| 소속 |
1연세대, 2서울대, 3분당서울대 |
| 키워드 |
Co-culture; Endothelial cells; Mesenchymal stem cells; Electrospun nanofiber; Angiogenesis
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| E-Mail |
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