화학공학소재연구정보센터
학회 한국화학공학회
학술대회 2008년 가을 (10/23 ~ 10/24, 부산 BEXCO)
권호 14권 2호, p.2533
발표분야 공정시스템
제목 μ-ELISA System for the Diagnosis of Hepatitis B Using Anodic Aluminum Oxide in a Micrufluidic System
초록 An efficient system for diagnosis of disease marker molecules in microfluidic device was developed by employing the anodic aluminum oxide (AAO). AAO was integrated into poly(dimethylsiloxane) (PDMS) microchannel structure. Aluminum layer was anodized by variation of electrolyte composition,applied voltage and time for specific pore sizes and depth. For the enhancement of antibody immobilization and adhesion with PDMS, surface activation of AAO was performed by TMOS-sol spin-coating and calcinations to form SiO2 layer. The demonstration of diagnosis of bio-marker protein was performed by employing conventional ELISA assay for hepatitis B virus. The anti-HBVsurface antigen(anti-HBsAg) was immobilized and coupled with HBsAg and sheep anti-HBs/horse radish peroxidase (HRP) conjugate. The identification was cleared by colorimetric assay in conformity with the result using commercial ELISA kit, with high efficiency using microfluidic channels. The AAO inside microfluidic channel allows specific immobilization of proteins by accessible size. Furthermore, this study can be carried out inhigh-throughput system for bio-marker.
저자 양광석1, 김혜진2, 안정근2, 김도현1
소속 1한국과학기술원, 2충남대
키워드 anodic aluminum oxide; ELISA; hepatitis B; Lab-on-a-chip
E-Mail DoHyun.Kim@kaist.ac.kr
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