| 학회 | 한국화학공학회 |
| 학술대회 | 2016년 가을 (10/19 ~ 10/21, 대전컨벤션센터) |
| 권호 | 22권 2호, p.1803 |
| 발표분야 | 생물화공 |
| 제목 | Enhanced in vivo diagnosis of cancer with multiple tumor targeting peptides presented proteinticle |
| 초록 | In this study, for enhanced cancer targeting, human ferritin heavy chain nanoparticle(hFTH) is engineered to present tumor-binding peptides(RGD-derived cyclic peptide(4CRGD) and/or affibody) on its surface. Affibody strongly binds to epidermal growth factor receptor 1(EGFR) and 4CRGD binds to human integrin αvβ3 that are overexpressed in various cancer cell lines. First through in vitro study with EGFR-overexpressing breast cancer cell(MDA-MB-468) and integrin-overexpressing glioblastoma cell(U87MG), it is shown that for active cancer targeting, specific interaction between receptor on tumor and tumor receptor binding peptides is important. After labeling these particles with the near-infrared fluorescence dye(Cy5.5) and intravenouse injection into MDA-MB-468 or U87MG tumor-bearing mice, the recombinant hFTHs that present each tumor-targeting peptide or both peptides are successfully delivered to cancer and showed prolonged retention in cancer. And for heterogeneous receptor expressing tumor(U87MG), the recombinant hFTH presenting both affibody and 4CRGD notably enhances in vivo detection. |
| 저자 | 조은지, 권구철, 이지원 |
| 소속 | 고려대 |
| 키워드 | 미생물공학; 바이오에너지/환경생물공학; 바이오칩/나노바이오공학; 기능성바이오/융합소재공학 |
| VOD | VOD 보기 |
| 원문파일 | 초록 보기 |
