| 학회 | 한국재료학회 |
| 학술대회 | 2021년 봄 (05/12 ~ 05/14, 광주 김대중컨벤션센터) |
| 권호 | 27권 1호 |
| 발표분야 | B. 나노화학/바이오 재료 분과 |
| 제목 | Insulin secretion analysis of Isolated pancreatic cells cultured on decellularized kidney Extracellular Matrix (ECM) |
| 초록 | Recently, extracellular matrix (ECM) has been attracted for regeneration of pancreas due to the increasing of end stage of diabetes type 1 patients. Pancreatic beta-cell is the main insulin secretion factory in the body and the fail of beta-cell induces the breakdown of controlling blood sugar system. Thus, diabetes disease confuses the reaction and operation of the overall body endocrine system, eventually leading to various complications in the human body. In an effort to treat diabetes, even though many researches have been reported concerning on the implantation of beta-cell into hepatic portal vein and under the renal capsule, but these approaches have been recognized as a temporal treatment. In this study, we made the isolation of pancreatic cell from rat pancreas. For the basic study on the pancreatic cell attachment and proliferation behavior, we made kidney ECM slice by the decellularization process and pancreatic cells were seeded on the decellularized kidney and pancreas ECM slices as well as 3D printed PCL scaffold as a negative control, to figure out the possibility of kidney ECM for artificial pancreas. For isolation of pancreatic cells, pancreas was extracted from Sprague dawley male rats and digested by collagenase P. Pancreatic islets were separated by histopaque concentration gradient and expanded in the developed pancreatic cell growth media. Expanded pancreatic cells were analyzed by flow cytometry and confirmed the existence of pancreatic beta cells. For the culturing of pancreatic cell on acellular scaffolds, rat kidneys and pancreas were extirpated from sprague dawley male rats and decellularized successfully with 1% SDS and 1% triton X-100. Decellularized scaffolds were analyzed by histopathological staining. Isolated pancreatic cells were cultured on the ECM slices and PCL scaffolds at 37℃ and 5% of CO2 humid conditions for 3 days, 1 week, and 2 weeks. Distribution of isolated pancreatic cells on kidney ECM as well as cell attachment and proliferation of beta cell were investigated by the fluorescence microscope and also bio-molecular analysis. |
| 저자 | Minji Choi1, Byong-Taek Lee2 |
| 소속 | 1Department of Regenerative Medicine, 2College of Medicine |
| 키워드 | Pancreatic cell; Kidney; Extracellular Matrix; Decellularization; Recellularization |
