| 초록 |
Although optical lithography or photolithography is one of the most well-established techniques for micro, nano-fabrication, its usage with proteins or cells is restricted since it must be carried out in harsh organic solvents. Here, we present a simple method to pattern fibronectin, an extracellular matrix protein that is involved in the adhesion and spreading of anchorage-dependent cells, using poly(dimethylsiloxane) (PDMS) as a mold. Poly(ethylene glycol) (PEG) was selected as biomaterial-nonfouling material, which provides a physico-chemical barrier to biomaterial attachment. We propose modified micromolding in capillary (MIMIC) method based on UV polymerization to obtain a surface of alternating PEG and fibronectin. First to fabricate PEG microstructure via MIMIC method, a pre-patterned PDMS mold is placed on the desired substrate, and then the relief structure in the mold forms a network of empty channels. A drop of ethylene glycol monomer solution containing the initiator for UV polymerization is placed at the open ends of the network of channels, which is then polymerized by exposure to UV light at room temperature. Once PEG microstructure is fabricated, incubation of the patterned surface in a fibronectin-containing solution allows back-filling of only the bare regions with fibronectin via adsorption. In the alternative method, a substrate is first incubated in a fibronectin-containing solution, leading to the adsorption of fibronectin over the entire surface, and the fibronectin-adsorbed substrate is then micropatterned with the PEG by MIMIC based on UV polymerization. Both methods create reproducible alternating PEG and fibronectin patterns applicable to cell-surface interactions on the microscale. |
