| 초록 |
The aim of this study was to develop a new gene carrier consisting of chitosan (CS), cystamine(CA), and PEI. We found that cystamine and PEI modified chitosan (CSCAP) can be deliver faster gene into nucleus of mammalian cell. Gelretardation assay showed that pDNA was completely condensed by CSCAP, and protected from DNase. Also, Its transfection was enhanced by proton sponge effect with modified PEI, and disulfide-bond of modified CA was dissociated by glutathione into cell. In addition, its cellular uptake was investigated by fluorescent microscope. Characterization of CSCAP was analysed by FT-IR, 1H-NMR, DLS and TEM. Moreover, Its cytotoxicity was confirmed by MTT assay. These results suggest that CSCAP can apply as a carrier of gene delivery. This work was supported by National Research Foundation of Korea (NRF) grant funded by the Ministry of Science, ICT & Future Planning. (No. NRF-2014R1A2A1A10053027). |
