| 학회 |
한국고분자학회 |
| 학술대회 |
2013년 가을 (10/11 ~ 10/12, 창원컨벤션센터) |
| 권호 |
38권 2호 |
| 발표분야 |
의료용 고분자 부문위원회 |
| 제목 |
Decreased cytotoxcity of PEI/pDNA polyplex with anionic polymer for gene delivery |
| 초록 |
Among non-viral vectors, polyethylenimine (PEI) is a popular cationic polymer due to high transfection efficiency. Poly (glutamic acid) (PGA) is a hydrophilic and anionic polymer with nontoxicity, biocompatibility, and nonimmunogenicity. To reduce high cytotoxicity of PEI, pDNA/PEI complex was surrounded with PGA. The size of ternary nanoparticle (PEI-pDNA-PGA) was measured by DLS. Its binding ability was determined by gel electrophoresis. In addition, gene transfection efficiency was evaluated by FACS, microscopy, and luciferase assay, resulted in effective vector on gene delivery system. This research was supported by Basic Science Research Program through the National Research Foundation of Korea(NRF) funded by the Ministry of Education, Science and Technology(2011-0012039) |
| 저자 |
박순정, 김준호, 김남홍, 김원석, 남정표, 최창용, 박성철, 나재운, 장미경
|
| 소속 |
순천대 |
| 키워드 |
gene carrier; GDS; glutamic acid; pDNA
|
| E-Mail |
|
