| 초록 |
A green solvents hybrid maccroporous molecularly imprinted silica monolithic column was synthesized by free radical polymerization within the confines of a stainless-steel column (50 mm × 4.6 mm id). In the processes, ionic liquid (1-allyl-3-butylimidazolium Br) and acrylamide were used as dual monomers, ionic liquid (1,2-bis [N-N’-vinyl imidazolium] ethane bis Br) and N,N’-methylenebisacrylamide as dual cross-linking agents, and DES (chcl: ethylene glycol 1:2) as porogen. Bovine serum albumin (BSA) and lysozyme (Lyz), which differ greatly in molecular size, were representatively selected for imprinted templates to evaluate recognition property of the green solvents hybrid MIP silica monolith. Under the optimum conditions, the obtained hybrid MIP silica monolith showed higher binding affinity for template than its corresponding non-imprinted (NIP) monolith. |
