| 초록 |
Microalgae have been considered as feedstocks for biofuel production. The green algae Chlamydomonas reinhardtii serves as an important model organism for studying perturbations in metabolic pathways under environmental stress conditions. C. reinhardtii accumulates lipids under nutrient limitation conditions. To understand biochemical changes under nutrient limitation, we used newly developed mutant C. reinhardtii with higher lipid content. Nitrogen NO3- and phosphate PO43- were used as stressor for nutrient starvation during the cultivation. Also, the lipid composition of C. reinhardtii mutant was investigated with thin-layer chromatography. Under nutrient starvation, total lipid content level was increased to 27~33% and C16:0 fatty acid content constituted over 31~43% of total fatty acid. Interestingly, we also found that the expression of fatty acid desaturase (FAD7) was decreased when nutrients were starved. |
