| 초록 |
In order to investigate biological mechanisms and interactions, the development of ultrasensitive biosensor has been necessary. Surface plasmon resonance (SPR) biosensor is one of the ultrasensitive biosensor platform which can be applied in real-time and label-free sensing area. A localized electromagnetic near-field, so called hot spot, is exploited for detection surface binding events. In this work, we designed a colocalization-based SPR sensor. The colocalization fabricated by hotspot-mediated plasmonic lithography represents an position matching of the analyte distribution and electromagnetic hot spot. The colocalization-based SPR sensor is proved to amplify the optical signal and thereby to achieve detection on the order of 100 streptavidin molecules with a binding capacity below 1 fg/mm2, and enhancement by more than three orders of magnitude in comparison with the conventional SPR detection. The effective limit of detection of colocalized measurements was on the order of 30 pM. |
