| 학회 |
한국공업화학회 |
| 학술대회 |
2020년 가을 (10/28 ~ 10/30, 광주 김대중컨벤션센터(Kimdaejung Convention Center)) |
| 권호 |
24권 1호 |
| 발표분야 |
포스터-생물공학 |
| 제목 |
Enhancing the tyosine production from acetate in Escherichia coli by optimization of glyoxylate cycle |
| 초록 |
L-tyrosine is a commercially important amino acid in industry. We attempt to control translation-level expression and carbon flux rebalancing around phosphoenolpyruvate (PEP) node. To optimize the L-tyrosine biosynthetic pathway, a synthetic constitutive promoter and a synthetic 5′-untranslated region (5′-UTR) were introduced for each gene of interest to allow for control at both transcription and translation levels. Carbon flux rebalancing was achieved by controlling the expression level of PEP synthetase using UTR Designer. The L-tyrosine productivity of the engineered E. coli strain was increased through pathway optimization resulting in 3.0g/L of L-tyrosine titer, 0.0354g L-tyrosine/h/g DCW of productivity, and 0.102g L-tyrosine/g glucose yield. Thus, this work demonstrates that pathway optimization by 5′-UTR redesign is an effective strategy for the development of L-tyrosine-producing bacteria. |
| 저자 |
조민지1, 노명현1, 강채원1, 임대균2, 오민규2, 정규열1
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| 소속 |
1포항공과대, 2고려대 |
| 키워드 |
Tyrosine; acetate; glyoxylate cycle; 5'-untranslated region
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| E-Mail |
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