| 학회 |
한국화학공학회 |
| 학술대회 |
2022년 봄 (04/20 ~ 04/23, 제주국제컨벤션센터) |
| 권호 |
28권 1호, p.938 |
| 발표분야 |
[주제 10] 헬스케어 |
| 제목 |
Encoded Hydrogel Microparticles Incorporated with Universal Mismatch DNA probes for Specific Detection of Multiple SNPs |
| 초록 |
Hydrogel microparticle-based nucleic acid assays are an attractive detection platform based on their multiplexing capabilities with high sensitivity. A particular area of interest is single-nucleotide polymorphism (SNP) sensing, where multiple SNPs should be identified in a highly reliable yet economical manner. However, hydrogel microparticles leveraging probe-target hybridization as a key mechanism are hampered by small duplex stability differences arising from single base-pair mismatch. We have developed encoded hydrogel microparticles with DNA probes tailored for multiplex SNP detection. Within the DNA probes, we adopt a widely used base analog so that it substitutes one of the base sequences among DNA probes. The effects of the probes modification on SNP sensing has been tested from specificity, sensitivity, and temperature window at a given ionic strength. We have validated that our hydrogel microparticles exhibit much higher specificity for a single base-pair mismatch with minimal reduction in sensitivity. Our particles can also detect multiple SNPs located in different target strands, which is a significant challenge for conventional particles. |
| 저자 |
문석준, 문현준, 이준호, 노윤호, 임용준, 봉기완
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| 소속 |
고려대 |
| 키워드 |
이동현상(Transport Phenomena) |
| E-Mail |
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| 원문파일 |
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