| 초록 |
Although optical lithography or photolithography is one of the most well-established techniques for micro-fabrication, its usage with proteins and cells is restricted by steps that must be carried out in harsh organic solvents. Here, we present a simple method for patterning protein and cell on poly(dimethylsiloxane) (PDMS). The substrate is partially modified with oxygen-plasma using a screen mask, therefore, the substrate is partitioned into regions of different hydrophilicities. The substrate consisting of two regions of different surface properties can be applicable for protein or cell patterning: the hydrophobic region that acts as a resistant layer and the hydrophilic region that promotes protein or cell absorption. Additionally, since the hydrophilic character of an oxygen-plasma modified PDMS surface is very unstable, we derivatized the oxygen-based plasma modified region of PDMS surface with hydrophilic polymer chains. Therefore, the PDMS substrate is more stably partitioned into two regions by hydrophilic polymer chain linkers. |
