| 학회 |
한국재료학회 |
| 학술대회 |
2013년 가을 (11/06 ~ 11/08, 제주롯데호텔) |
| 권호 |
19권 2호 |
| 발표분야 |
D. 바이오재료(Biomaterials) |
| 제목 |
Material characterization and In-Vitro biocompatibility of Sponge scaffolds with In-vivo deposited Extra Cellular Matrix (ECM) for Bone Tissue engineering. |
| 초록 |
This study aims to produce a sponge scaffold with inherent extracellular matrix (ECM) for bone tissue regeneration without employing the usual hydrogel coating or loading method. To incorporate the ECM to the scaffold BCP or HAP, samples of the scaffolds measuring 1 x 1 cm and 0.5cm thick were implanted in the subcutaneous pockets of rats and fibrous tissue was allowed to propagate for up to two weeks. Implanted samples were then extracted and decellularized using either 1% Triton X with ammonium hydroxide or 1% sodium dodecyl disulfate (SDS). Material properties such as compressive strength and porosity was observed from samples after extraction and after decellularization. Morphology of the extracted and decellularized samples were also observed through scanning electron morphology and histological sections and compared with the original unmodified scaffold. In vitro biocompatibility of the decellularized constructs was determined by seeding MC3T3-E1 pre- osteoblast cells and observing cell proliferation using MTT assay and cell adhesion behaviour through confocal microspcopy one week after seeding. |
| 저자 |
Reiza D. Ventura1, Andrew R. Padalhin2, Young-Ki Min3, Byong-Taek Lee4
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| 소속 |
1Department of Regenerative Medicine, 2College of Medicine, 3Soonchunhyang Univ., 4Cheonan |
| 키워드 |
sponge scaffold; extracellular matrix; bone regeneration; decellularization
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| E-Mail |
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