| 학회 |
한국고분자학회 |
| 학술대회 |
2018년 봄 (04/04 ~ 04/06, 대전컨벤션센터) |
| 권호 |
43권 1호 |
| 발표분야 |
고분자가공/복합재료 |
| 제목 |
Polymer layered gold nanoparticle for gene delivery by SI-ATRP |
| 초록 |
Gold nanoparticles were surface-decorated with densely-packed anionic shells by surface initiated atom transfer radical polymerization (SI-ATRP) and assembled siRNA/PEI layers for enhanced incorporation of nucleic acids. Synthesized AuNP showed a size of 12nm by TEM. After SI-ATRP of SEMA, the diameter was increased up 24 nm by TEM, suggesting that pSEMA shells were decorated on AuNP. After immobilization of pSEMA, PEI and siRNA were incorporated by electrostatic interactions. These carriers showed increased cationic charge densities on the surface, suggesting siRNA was incorporated on the surface according the various charge ratio (nitrogen/phosphate). TEM-elemental mapping clearly confirmed the presence of pSEMA, PEI, and siRNA on the surface of AuNP. Since we successfully incorporated siRNA on the bi-layers of PEI and pSEMA shells, these carrier showed different incorporation efficiency according to the charge ratio of PEI/siRNA, which was confirmed by gel retardation assay. |
| 저자 |
이솔, 유혁상, 손영주, Wei Mao, 신지운, 이주원
|
| 소속 |
강원대 |
| 키워드 |
gold nanoparticle; SI-ATRP; layer by layer; gene delivery
|
| E-Mail |
|
