| 초록 |
Tissue adhesives are required to perform a variety of functions including sealing leaks, stop bleeding, binding tissues, and preferably facilitating a healing process. Chitosan is a cationic polymer, known to have a hemostatic property, biocompatibility and tissue adhesion ability. In this study, we prepared chitosan-based in situ forming hydrogels (CPT) via enzymatic crosslinking reaction as hemostat and wound closure. The CPT conjugates were synthesized by urethane linkage chemistry. The hydrogels were formed with horseradish peroxidase and H2O2. The physico-chemical properties of the hydrogels such as gelation time, elastic moduli, and adhesive strengths were characterized under various conditions. The in vivo study showed that the hydrogels had excellent hemostatic and adhesive properties as well as the wound healing capability. Obtained results suggest that the in situ forming chitosan hydrogels are promising biomaterials for tissue engineering in surgical applications. |
